Data/hora: 12/05/2024 01:37:46

Biblioteca(s): 	Embrapa Suínos e Aves.	
Data corrente: 	19/11/2014	
Data da última atualização: 	19/11/2014	
Autoria: 	VARELA, A. P. M.; SANTOS, H. F. dos; CIBULSKI, S. P.; SCHEFFER, C. M.; SCHMIDT, C.; LIMA, F. E. S.; SILVA, A. D.; ESTEVES, P. A.; FRANCO, A. C.; ROEHE, P. M.	
Afiliação: 	ANA PAULA MUTERLE VARELA, FEPAGRO; HELTON FERNANDES DOS SANTOS, FEPAGRO; SAMUEL PAULO CIBULSKI, FEPAGRO; CAMILA MENGUE SCHEFFER, FEPAGRO; CANDICE SCHMIDT, FEPAGRO; FRANCISCO ESMAILE SALES LIMA, UFGRS; ALESSANDRA D'ÁVILA SILVA; PAULO AUGUSTO ESTEVES, CNPSA; ANA CLÁUDIA FRANCO, UFGRS; PAULO MICHEL ROEHE, FEPAGRO.	
Título: 	Chicken anemia virus and avian gyrovirus 2 as contaminants in poultry vaccines.	
Ano de publicação: 	2014	
Fonte/Imprenta: 	Biologicals, London, v. 42, n. 6., p. 346–350, 2014.	
Idioma: 	Inglês	
Conteúdo: 	This study focuses on the detection of chicken anemia virus (CAV) and avian gyrovirus 2 (AGV2) genomes in commercially available poultry vaccines. A duplex quantitative real-time PCR (dqPCR), capable of identifying genomes of both viruses in a single assay, was employed to determine the viral loads of these agents in commercially available vaccines. Thirty five vaccines from eight manufacturers (32 prepared with live and 3 with inactivated microorganisms) were examined. Genomes of CAV were detected as contaminants in 6/32 live vaccines and in 1/3 inactivated vaccines. The CAV genome loads ranged from 6.4 to 173.4 per 50 ng of vaccine DNA (equivalent to 0.07 to 0.69 genome copies per dose of vaccine). Likewise, AGV2 genomes were detected in 9/32 live vaccines, with viral loads ranging from 93 to 156,187 per 50 ng of vaccine DNA (equivalent to 0.28e9176 genome copies per dose of vaccine). These findings provide evidence for the possibility of contamination of poultry vaccines with CAV and AGV2 and they also emphasize the need of searching for these agents in vaccines in order to ensure the absence of such potential contaminants.	
Thesagro: 	Anemia infecciosa; Ave doméstica; Frango; Virologia.	
Thesaurus Nal: 	Chicken anemia virus; Gyrovirus; Live vaccines; Poultry; Virology.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Suínos e Aves (CNPSA)	
Exemplares: 	CNPSA - (19815-1) - UPC - AP - DD -  -  -  - DIS